2.A.1 Name of plan "owner" (should be faculty PI)Ihab Younis
2.A.2 CMU-Q email of plan ownerEmail hidden; Javascript is required.
2.A.3 Department of plan ownerQBS
2.B Justification of on-site work

I already submitted a plan that was approved. In the original plan I described the need for on-site research as we have done exhaustive computational analysis and it is paramount that we now do the wet lab experiments to move the research projects forward. This applies to both Seed grant and NPRP10 grant.
I am submitting an updated plan because I need to add a student to my plan. The student is Mariam El-Esnawy (Andrew ID is melesnaw) who is currently a senior in Biological Sciences Program at CMUQ. She is doing research as part of CMUQ-funded project (QSIURP). She started working on the computational aspect of her research 3 weeks ago and needs to also start the in-person lab experimentation after Fall break (October 18th). She completed her return to campus training on Canvas and will be added to the Research Activity Planner calendar so that she can sign in daily to track where she is in the building.

2.C.1 Facility details and locations
Room No. Location name/description
3027 Research laboratory on third floor
3025 Teaching laboratory on third floor
3070 Ihab Younis Office
3022 Research laboratory on second floor
3142 BSL2 laboratory on third floor
3222 Storage room on third floor
2.C.2 Personnel details
Name AndrewID Personnel type (faculty, staff, student) Relevant spaces to access # Level of access ##
Ihab Younis iyounis faculty all A
Mariam El-Esnawy melesnaw student 3027, 3025 B
2.C.3 Acknowledge that people listed above have been consulted
  • Yes, people listed above have been consulted
2.D Pandemic Safety Officer
PSO Name PSO AndrewID Date of training #
Ihab Younis iyounis July 14, 2020
2.E.1 Short descriptoin of project(s) and type(s) of work

Seed Project: The role of alternative pre-mRNA splicing in DNA damage repair and breast cancer.
NPRP10-0117-170178 Project: Molecular Profiling of Breast Cancer Transcriptome and Splicing Aberrations.
For both projects the following experiments would be performed:
-Tissue culture of breast cancer cells
-Treating cells with splicing-modifying oligonucleotides
-RNA extraction, reverse transcription, and PCR
-Protein extraction, SDS-PAGE, and western blotting
-Cloning of RNA binding proteins using PCR, transformation of bacteria cells, minipreps and midipreps.

2.E.2 Does your work involve human subjects?No
2.E.3 If not requesting ongoing access, list date ranges for which you are requesting access

ongoing access for Ihab Younis
October 18, 2020 till April 30, 2020 for Mariam ElEsnawy. Mariam's dates are as such because her project was originally slated for Summer 2020 and was pushed due to COVID-19 and now is spread over two semesters and winter break.

2.F Prioritization considerations

For Ihab Younis, a progress report for his NPRP10-0117-170178 Project is due on December 3, 2020. His presence in the lab is required to perform the experiments that were outlined in his QNRF application.
For Mariam ElEsnawy, her QSIURP project was awarded Spring 2020 and was supposed to start Summer 2020 (Full time). The QSIURP approved delaying the start of her project but to complete all the requirements she needs to start after Fall break and keep working part-time on the project for around 30 weeks.

2.G.1.a Describe any scheduling tools you will use to control access to particular rooms and appratus?

All members of the plan will strictly abide by the IT-generated Research Activity Planner calendar.

2.G.1.b If you intend to use pre-defined rotations or shifts, attach (at end) or provide below an example schedule

Since Mariam is an undergraduate student and cannot be in the laboratory alone, Ihab younis schedule will match hers. She is assigned to be present in rooms where the max occupancy is at least 4 persons.

2.G.1.c Are there any circumstances where personnel CANNOT maintain physical distancing for short periods of time?No
2.G.2 Anyone who is sick must stay home
  • Yes, I affirm
2.G.3 Face masks
  • Yes, I affirm
2.G.4 Individual hygiene & hand-washing
  • Yes, I affirm
2.G.5 Facility entrance/exit logging processes

All personnel will log in using the IT-generated Research Activity Planner calendar.

3 Ramp-down and temporary suspension of work plans

In case a lab shut down is ordered due to infection spread or emergency, the following shut down plan will be executed:
• Ensure freezers (-20C & -80C degrees) and fridges are connected to emergency power.
• Ensure that one fume cupboard is connected to emergency power
• Samples and chemicals in the freezers that are temperature sensitive will remain in freezers with emergency backup.
• Actively growing cultures will be placed immediately into fridges; if opportunity to freeze (time permission) this will be done.
• All equipment (with the exception of freezers and fridges) will be disconnected from power supply; especially heat equipment like water bath, heating blocks and ovens
• Bench-tops will be cleared and wiped down and freed of clutter
• All personal belongings will be removed from labs
• Glass bottles or reagents (at room temperature) will be stored away into cupboards.
• Volatile chemicals will be stored away in the respective fume cupboards, all flammables will be stored away into flammable cabinets
• Chemical waste will collect in one satellite collection point – this fume cupboard should be connected to the emergency power outlet
• Accumulated waste will be autoclaved (if time permits)

4 Regular reporting details
  • Yes, I acknowledge
5 Acknowledgement of positive COVID-19 diagnosis response protocols and temporary work suspension
  • Yes, I acknowledge
6.b Attachments